lipofectamine rnaimax (Santa Cruz Biotechnology)
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Lipofectamine Rnaimax, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 6363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine+rnaimax/Control+siRNA-A/pm41595728-79-19-15
Average 96 stars, based on 6363 article reviews
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Transfection:Article Title: Increased circulating miR-370-3p regulates steroidogenic factor 1 in endometriosis Article Snippet: .. Briefly, cells were transfected at 65% confluence in a six-well tissue culture plates with either SF-1 plasmid (no. sc-402334-ACT, 10 μg/ml) and hsa-miR-370-3p mimic together or siRNA plasmid (no. sc-402334, 1 μg/ml) later with hsa-miR-370-3p mimic using Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis. Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Article Title: miR-222-3p is involved in neural tube closure by directly targeting Ddit4 in RA induced NTDs mouse model. Article Snippet: 5 × 105 cells/ ml was plated in antibiotic-free medium for 24 h before transfections. miR-222-3p mimics (mim222), miR-222-3p inhibitor (anti-222), miR mimics negative control (mim-NC), miR inhibitor negative control (anti-NC) and siRNA-Ddit4 plasmid were designed and synthesized by GenePharma Co (Shanghai, China). .. When the HT22 cells become 75–85% confluent, cells were transiently transfected with 100 nM miRNA or siRNA using Article Title: The Reciprocal Causation of the ASK1-JNK1/2 Pathway and Endoplasmic Reticulum Stress in Diabetes-Induced Cognitive Decline Article Snippet: CCK8 assay (Beyotime, Shanghai, China) was used to detect the optimum concentration of HG. .. After seeding for 24 h, the cells were cultured in HG (100 mM) media with or without 2 mmol/L ER stress inhibitor 4-phenylbutyric acid (4-PBA) to inhibit ER stress for 24 h. Additionally, ASK1 small interfering RNA (ASK1-siRNA; sc-29749, Santa Cruz Biotechnology, CA, United States) and control siRNA (sc-37007) were used to inhibit ASK1 expression in cells for 24 h. Article Title: Thrombospondin-1 Receptor CD47 Overexpression Contributes to P-Glycoprotein-Mediated Multidrug Resistance Against Doxorubicin in Thyroid Carcinoma FTC-133 Cells Article Snippet: .. FTC-133R cells were transfected with non-targeting control scRNA (scRNA-cells) or siRNA specific to CD-47 (siRNA-cells) using Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Article Title: The ANGPTL3-integrin α5 axis drives retinal vascular leakage in diabetic retinopathy. Article Snippet: The precleared lysates were incubated overnight at 4°C with gentle rotation in the presence of anti-ANGPTL3 (Thermo Fisher Scientific, Cat# MA5-35681, RRID: AB_2849581), antiintegrin α5 (Abcam, Cat# ab179475, RRID: AB_2716738), or antiIgG. .. The samples were separated by SDS-PAGE, and immunoprecipitated proteins were detected by immunoblotting using appropriate secondary antibodies. siRNA transfection HUVECs at 60% confluence were transfected with 50 nmol/L of integrin α5-specific siRNA (Santa Cruz Biotechnology, Cat# sc29372) or a scrambled control siRNA using Plasmid Preparation:Article Title: Increased circulating miR-370-3p regulates steroidogenic factor 1 in endometriosis Article Snippet: .. Briefly, cells were transfected at 65% confluence in a six-well tissue culture plates with either SF-1 plasmid (no. sc-402334-ACT, 10 μg/ml) and hsa-miR-370-3p mimic together or siRNA plasmid (no. sc-402334, 1 μg/ml) later with hsa-miR-370-3p mimic using Article Title: Ethionine-mediated reduction of S -adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway Article Snippet: The cells were treated with 20 mmol/L ethionine, 2 mmol/L SAM for 48 h. The siRNA-METTL3 plasmid was synthesized by Sangon Biotech (Shanghai, China). .. The METTL3 plasmid was synthesized by Gene Pharma (Shanghai, China), and transfected into the cells using Control:Article Title: Increased circulating miR-370-3p regulates steroidogenic factor 1 in endometriosis Article Snippet: .. Briefly, cells were transfected at 65% confluence in a six-well tissue culture plates with either SF-1 plasmid (no. sc-402334-ACT, 10 μg/ml) and hsa-miR-370-3p mimic together or siRNA plasmid (no. sc-402334, 1 μg/ml) later with hsa-miR-370-3p mimic using Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis. Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Article Title: The Reciprocal Causation of the ASK1-JNK1/2 Pathway and Endoplasmic Reticulum Stress in Diabetes-Induced Cognitive Decline Article Snippet: CCK8 assay (Beyotime, Shanghai, China) was used to detect the optimum concentration of HG. .. After seeding for 24 h, the cells were cultured in HG (100 mM) media with or without 2 mmol/L ER stress inhibitor 4-phenylbutyric acid (4-PBA) to inhibit ER stress for 24 h. Additionally, ASK1 small interfering RNA (ASK1-siRNA; sc-29749, Santa Cruz Biotechnology, CA, United States) and control siRNA (sc-37007) were used to inhibit ASK1 expression in cells for 24 h. Article Title: Thrombospondin-1 Receptor CD47 Overexpression Contributes to P-Glycoprotein-Mediated Multidrug Resistance Against Doxorubicin in Thyroid Carcinoma FTC-133 Cells Article Snippet: .. FTC-133R cells were transfected with non-targeting control scRNA (scRNA-cells) or siRNA specific to CD-47 (siRNA-cells) using Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Article Title: The ANGPTL3-integrin α5 axis drives retinal vascular leakage in diabetic retinopathy. Article Snippet: The precleared lysates were incubated overnight at 4°C with gentle rotation in the presence of anti-ANGPTL3 (Thermo Fisher Scientific, Cat# MA5-35681, RRID: AB_2849581), antiintegrin α5 (Abcam, Cat# ab179475, RRID: AB_2716738), or antiIgG. .. The samples were separated by SDS-PAGE, and immunoprecipitated proteins were detected by immunoblotting using appropriate secondary antibodies. siRNA transfection HUVECs at 60% confluence were transfected with 50 nmol/L of integrin α5-specific siRNA (Santa Cruz Biotechnology, Cat# sc29372) or a scrambled control siRNA using Over Expression:Article Title: Increased circulating miR-370-3p regulates steroidogenic factor 1 in endometriosis Article Snippet: .. Briefly, cells were transfected at 65% confluence in a six-well tissue culture plates with either SF-1 plasmid (no. sc-402334-ACT, 10 μg/ml) and hsa-miR-370-3p mimic together or siRNA plasmid (no. sc-402334, 1 μg/ml) later with hsa-miR-370-3p mimic using Negative Control:Article Title: Increased circulating miR-370-3p regulates steroidogenic factor 1 in endometriosis Article Snippet: .. Briefly, cells were transfected at 65% confluence in a six-well tissue culture plates with either SF-1 plasmid (no. sc-402334-ACT, 10 μg/ml) and hsa-miR-370-3p mimic together or siRNA plasmid (no. sc-402334, 1 μg/ml) later with hsa-miR-370-3p mimic using Cell Culture:Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis. Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Article Title: The Reciprocal Causation of the ASK1-JNK1/2 Pathway and Endoplasmic Reticulum Stress in Diabetes-Induced Cognitive Decline Article Snippet: CCK8 assay (Beyotime, Shanghai, China) was used to detect the optimum concentration of HG. .. After seeding for 24 h, the cells were cultured in HG (100 mM) media with or without 2 mmol/L ER stress inhibitor 4-phenylbutyric acid (4-PBA) to inhibit ER stress for 24 h. Additionally, ASK1 small interfering RNA (ASK1-siRNA; sc-29749, Santa Cruz Biotechnology, CA, United States) and control siRNA (sc-37007) were used to inhibit ASK1 expression in cells for 24 h. Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Knockdown:Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis. Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Article Title: Acacetin Attenuates Lysophosphatidylcholine-Induced Vascular Smooth Muscle Cell Injury via Sirt1-Nrf2/p62 Signaling Axis Article Snippet: .. For siRNA-mediated gene silencing experiments, cells transfected with Nrf2-targeting siRNA (50 nM) or scramble control (Santa Cruz, sc-37007) using Synthesized:Article Title: Ethionine-mediated reduction of S -adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway Article Snippet: The cells were treated with 20 mmol/L ethionine, 2 mmol/L SAM for 48 h. The siRNA-METTL3 plasmid was synthesized by Sangon Biotech (Shanghai, China). .. The METTL3 plasmid was synthesized by Gene Pharma (Shanghai, China), and transfected into the cells using Small Interfering RNA:Article Title: The Reciprocal Causation of the ASK1-JNK1/2 Pathway and Endoplasmic Reticulum Stress in Diabetes-Induced Cognitive Decline Article Snippet: CCK8 assay (Beyotime, Shanghai, China) was used to detect the optimum concentration of HG. .. After seeding for 24 h, the cells were cultured in HG (100 mM) media with or without 2 mmol/L ER stress inhibitor 4-phenylbutyric acid (4-PBA) to inhibit ER stress for 24 h. Additionally, ASK1 small interfering RNA (ASK1-siRNA; sc-29749, Santa Cruz Biotechnology, CA, United States) and control siRNA (sc-37007) were used to inhibit ASK1 expression in cells for 24 h. Expressing:Article Title: The Reciprocal Causation of the ASK1-JNK1/2 Pathway and Endoplasmic Reticulum Stress in Diabetes-Induced Cognitive Decline Article Snippet: CCK8 assay (Beyotime, Shanghai, China) was used to detect the optimum concentration of HG. .. After seeding for 24 h, the cells were cultured in HG (100 mM) media with or without 2 mmol/L ER stress inhibitor 4-phenylbutyric acid (4-PBA) to inhibit ER stress for 24 h. Additionally, ASK1 small interfering RNA (ASK1-siRNA; sc-29749, Santa Cruz Biotechnology, CA, United States) and control siRNA (sc-37007) were used to inhibit ASK1 expression in cells for 24 h. Immunoprecipitation:Article Title: The ANGPTL3-integrin α5 axis drives retinal vascular leakage in diabetic retinopathy. Article Snippet: The precleared lysates were incubated overnight at 4°C with gentle rotation in the presence of anti-ANGPTL3 (Thermo Fisher Scientific, Cat# MA5-35681, RRID: AB_2849581), antiintegrin α5 (Abcam, Cat# ab179475, RRID: AB_2716738), or antiIgG. .. The samples were separated by SDS-PAGE, and immunoprecipitated proteins were detected by immunoblotting using appropriate secondary antibodies. siRNA transfection HUVECs at 60% confluence were transfected with 50 nmol/L of integrin α5-specific siRNA (Santa Cruz Biotechnology, Cat# sc29372) or a scrambled control siRNA using Western Blot:Article Title: The ANGPTL3-integrin α5 axis drives retinal vascular leakage in diabetic retinopathy. Article Snippet: The precleared lysates were incubated overnight at 4°C with gentle rotation in the presence of anti-ANGPTL3 (Thermo Fisher Scientific, Cat# MA5-35681, RRID: AB_2849581), antiintegrin α5 (Abcam, Cat# ab179475, RRID: AB_2716738), or antiIgG. .. The samples were separated by SDS-PAGE, and immunoprecipitated proteins were detected by immunoblotting using appropriate secondary antibodies. siRNA transfection HUVECs at 60% confluence were transfected with 50 nmol/L of integrin α5-specific siRNA (Santa Cruz Biotechnology, Cat# sc29372) or a scrambled control siRNA using |
